The biological identity of nanoparticles (NPs) is defined by a protein layer formed on their surface, called protein corona (PC), once they meet the biological milieu. Any change in the PC composition may influence the biological fate of NPs. The PC composition is strongly dependent on several parameters including the physicochemical properties of NPs, and biological and environmental factors. As one of the main features of plasmonic NPs is their capacity to induce local heating by laser irradiation, we hypothesized that laser irradiation may change the biological identity of NPs and therefore alter their biological fate. To test this hypothesis, here we investigated the effects of either simultaneous or sequential laser irradiation on the conformations of a few proteins selected from two main categories of plasma proteins (i.e. human serum albumin and human fibrinogen) on the surfaces of gold nanorods (AuNRs). The outcomes revealed a significant role of laser irradiation on conformational changes of fibrinogen compared to albumin. Moreover, the effects of plasmonic heating - at various times - on the achieved corona composition from interactions of AuNRs and human plasma with various concentrations were monitored. Consequently, the cellular uptake of the corona coated AuNRs was measured in two cell types: malignant (MCF-7) and normal (MCF-10A) breast cell lines. The results demonstrated a substantial reduction in the cellular uptake of AuNRs in response to an increase in the laser irradiation time, especially in MCF-10A. Our results may pave the way for a mechanistic understanding of the biological identity of plasmonic NPs which in turn can help their safe and efficient clinical translations.